Journal: Breast Cancer : Targets and Therapy

Article Title: Anticarcinogenic Effects of Odorant Substances Citral, Citrathal R and Cyclovertal on Breast Cancer in vitro

doi: 10.2147/BCTT.S322619

Figure Lengend Snippet: MTT-assay. ( A ) MTT-assay T-47D cells, ( B ) MTT-assay BT474 cells, ( C ) MTT-assay MDA-MB-231 cells.

Article Snippet: BT474 cells (#300131) and T-47D cells (#300353) were purchased from CLS Cell Lines Service GmbH (Eppelheim, Germany), MDA-MB-231 cells (#92020424) from Sigma-Aldrich (Taufkirchen, Germany).

Techniques: MTT Assay

Journal: Breast Cancer : Targets and Therapy

Article Title: Anticarcinogenic Effects of Odorant Substances Citral, Citrathal R and Cyclovertal on Breast Cancer in vitro

doi: 10.2147/BCTT.S322619

Figure Lengend Snippet: Ki67 in T-47D, MDA-MB-231 and BT474 cells. Relative intracellular expression of Ki67 RNA in T-47D cells ( A ), MDA-MB-231 cells ( B ) and BT474 cells ( C ) after treatment with 50µM citral (Cit), 50µM citrathal R (CR) and 50µM Cyclovertal (Cy). CTRL: DMSO-Control.

Article Snippet: BT474 cells (#300131) and T-47D cells (#300353) were purchased from CLS Cell Lines Service GmbH (Eppelheim, Germany), MDA-MB-231 cells (#92020424) from Sigma-Aldrich (Taufkirchen, Germany).

Techniques: Expressing

Journal: Breast Cancer : Targets and Therapy

Article Title: Anticarcinogenic Effects of Odorant Substances Citral, Citrathal R and Cyclovertal on Breast Cancer in vitro

doi: 10.2147/BCTT.S322619

Figure Lengend Snippet: Survivin in T-47D, MDA-MB-231 and BT474 cells. Relative intracellular expression of survivin RNA in T-47D cells ( A ), MDA-MB-231 cells ( B ) and BT474 cells ( C ) after treatment with 50µM citral (Cit), 50µM citrathal R (CR) and 50µM Cyclovertal (Cy). CTRL: DMSO-Control.

Article Snippet: BT474 cells (#300131) and T-47D cells (#300353) were purchased from CLS Cell Lines Service GmbH (Eppelheim, Germany), MDA-MB-231 cells (#92020424) from Sigma-Aldrich (Taufkirchen, Germany).

Techniques: Expressing

Journal: Breast Cancer : Targets and Therapy

Article Title: Anticarcinogenic Effects of Odorant Substances Citral, Citrathal R and Cyclovertal on Breast Cancer in vitro

doi: 10.2147/BCTT.S322619

Figure Lengend Snippet: Cyclin A2 in T47-D, MDA-MB-231 and BT474 cells. Relative intracellular expression of cyclin A2 RNA in T-47D cells ( A ), MDA-MB-231 cells ( B ) and BT474 cells ( C ) after treatment with 50µM citral (Cit), 50µM citrathal R (CR) and 50µM Cyclovertal (Cy). CTRL: DMSO-Control.

Article Snippet: BT474 cells (#300131) and T-47D cells (#300353) were purchased from CLS Cell Lines Service GmbH (Eppelheim, Germany), MDA-MB-231 cells (#92020424) from Sigma-Aldrich (Taufkirchen, Germany).

Techniques: Expressing

Journal: Breast Cancer : Targets and Therapy

Article Title: Anticarcinogenic Effects of Odorant Substances Citral, Citrathal R and Cyclovertal on Breast Cancer in vitro

doi: 10.2147/BCTT.S322619

Figure Lengend Snippet: Western blots. Western Blot showing control, capsazepine alone, citral, citral + capsazepine, citrathal R, citrathal R + capsazepine, cyclovertal and cyclovertal + capsazepine. ß-tubulin served as loading control in ( A ) T-47D, ( B ) BT474, ( C ) MDA-MB-231.

Article Snippet: BT474 cells (#300131) and T-47D cells (#300353) were purchased from CLS Cell Lines Service GmbH (Eppelheim, Germany), MDA-MB-231 cells (#92020424) from Sigma-Aldrich (Taufkirchen, Germany).

Techniques: Western Blot

Journal: Breast Cancer : Targets and Therapy

Article Title: Anticarcinogenic Effects of Odorant Substances Citral, Citrathal R and Cyclovertal on Breast Cancer in vitro

doi: 10.2147/BCTT.S322619

Figure Lengend Snippet: Immunocytochemistry. 1: T-47D PCNA. Immunocytochemical staining of T47D cells with PCNA antibody (dilution 1:7500, microscopic enlargement 400x). (a) control, (b) citral, (c) citrathal R, (d) cyclovertal. Compared to control reduced nuclear–cytoplasmic ratio and increased nuclear condensation. 2: T-47D Annexin V. Immunocytochemical staining of T47D cells with Annexin V antibody (dilution 1:250, microscopic enlargement 400x). (a) control, (b) citral, (c) citrathal R, (d) cyclovertal. Compared to control reduced nuclear–cytoplasmic ratio and increased nuclear condensation. Slightly increased staining of the outer cell membrane after citral treatment, more after citrathal R and cyclovertal, compared to the control. 3: BT474 PCNA. Immunocytochemical staining of BT474 cells with PCNA antibody (dilution 1:7500, microscopic enlargement 400x). (a) control, (b) citral, (c) citrathal R, (d) cyclovertal. Compared to control reduced nuclear–cytoplasmic ratio and increased nuclear condensation. 4: BT474 Annexin V. Immunocytochemical staining of BT474 cells with Annexin V antibody (dilution 1:250, microscopic enlargement 400x). (a) control, (b) citral, (c) citrathal R, (d) cyclovertal. Clearly increased staining of the outer cell membrane after citral, cylovertal and mostly after citrathal R treatment compared to the control. 5: MDA-MB-231 PCNA. Immunocytochemical staining of MDA-MB-231 cells with PCNA antibody (dilution 1:7500, microscopic enlargement 400x). (a) control, (b) citral, (c) citrathal R, (d) cyclovertal. Compared to control reduced condensation and increased fragmentation of the nucleus and loss of spindle-shape after treatment mostly with citral and citrathal R, but also cyclovertal. Less intensive staining of the nuclei after treatment with citral, citrathal R and cyclovertal compared to the control. 6: MDA-MB-231 Annexin V. Immunohistochemical staining of MDA-MB-231 cells with Annexin V antibody (dilution 1:250, microscopic enlargement 400x). (a) control, (b) citral, (c) citrathal R, (d) cyclovertal. Compared to control reduced condensation and increased fragmentation of the nucleus and loss of spindle-shape after treatment with citral, citrathal R and cyclovertal. Increased staining of the outer cell membrane after citral, citrathal R and mostly after cyclovertal treatment compared to the control.

Article Snippet: BT474 cells (#300131) and T-47D cells (#300353) were purchased from CLS Cell Lines Service GmbH (Eppelheim, Germany), MDA-MB-231 cells (#92020424) from Sigma-Aldrich (Taufkirchen, Germany).

Techniques: Immunocytochemistry, Staining, Immunohistochemical staining

Journal: Nucleic acids research

Article Title: Spatially multiplexed RNA in situ hybridization to reveal tumor heterogeneity.

doi: 10.1093/nar/gkz1151

Figure Lengend Snippet: Figure 1. Local RNA in situ hybridization. (A) Schematic overview of a microfluidic implementation of chromogenic RNA in situ hybridization. The initial preparation of the tissue section including paraffin removal (1), heat (2) and protease (3) pretreatment, as well as fixation was performed globally. The primary probe was then hybridized locally to the RNA of interest (4). In this case the detection of HER2 is shown with internal negative (dapB) and positive (ActB) controls. Finally, the amplification of the signal using preamplifier and amplifier strands (5), the binding of enzyme (6), and the enzymatic reactions (7) were performed globally on the whole slide. (B) Fluorescence images of RNA-ISH experiments detecting the gene expression of HER2 in cell pellet sections of the breast cancer cell lines MCF7 (left), SKBR3 (center) and BT474 (right). Probes directed against bacterial dapB and ActB were used as negative and positive controls, respectively. Scale bar: 100 m.

Article Snippet: FFPE cell blocks and tissues FFPE cell blocks of the cell lines MCF7, BT474 and SKBR3 (AMS Biotechnology, Abingdon, UK) with HER2 expression levels of 1+, 2+ and 3+, respectively, were used as references.

Techniques: RNA In Situ Hybridization, Amplification, Binding Assay, Fluorescence, Gene Expression

Journal: Nucleic acids research

Article Title: Spatially multiplexed RNA in situ hybridization to reveal tumor heterogeneity.

doi: 10.1093/nar/gkz1151

Figure Lengend Snippet: Figure 2. Detection of HER2 expression status by RNA in situ hybridization using internal positive and negative controls. (A) Fluorescence images of the signals detected for HER2, dapB, and ActB in a mammary carcinoma section. Scale bar: 100 m. (B) Quantitative analysis of the fluorescence intensity of FISH signals detected for HER2, dapB, and ActB integrated per sectioned cell for FFPE sections of the breast cancer cell lines MCF7, SKBR3, and BT474 (see Figure 1) as well as the mammary carcinoma from (A). 100 ms excitation.

Article Snippet: FFPE cell blocks and tissues FFPE cell blocks of the cell lines MCF7, BT474 and SKBR3 (AMS Biotechnology, Abingdon, UK) with HER2 expression levels of 1+, 2+ and 3+, respectively, were used as references.

Techniques: Expressing, RNA In Situ Hybridization, Fluorescence

Journal: Nucleic acids research

Article Title: Spatially multiplexed RNA in situ hybridization to reveal tumor heterogeneity.

doi: 10.1093/nar/gkz1151

Figure Lengend Snippet: Figure 3. Single color multiplexed RNA-ISH for the detection of breast cancer biomarkers. (A) The primary probes for ER, PgR, and HER2 were delivered to spatially distinct regions of a mammary carcinoma section using a microfluidic chip (schematic in upper left corner). Fluorescence images of the signal detected for the breast cancer biomarkers ER, PgR and HER2 in a mammary carcinoma section. Scale bar: 100 m. (B) Fluorescence intensity of FISH signals detected for ER, PgR and HER2 integrated per sectioned cell for FFPE sections of the breast cancer cell lines MCF7, SKBR3 and BT474 (see Supplementary Figure S7) as well as the mammary carcinoma from (A). 500 ms excitation.

Article Snippet: FFPE cell blocks and tissues FFPE cell blocks of the cell lines MCF7, BT474 and SKBR3 (AMS Biotechnology, Abingdon, UK) with HER2 expression levels of 1+, 2+ and 3+, respectively, were used as references.

Techniques: Fluorescence

Journal: Journal for Immunotherapy of Cancer

Article Title: Preconditioning of the tumor microenvironment with oncolytic reovirus converts CD3-bispecific antibody treatment into effective immunotherapy

doi: 10.1136/jitc-2020-001191

Figure Lengend Snippet: Combination of reovirus and CD3-bispecific antibodies (bsAbs) is effective in a human, orthotopic human epidermal growth factor receptor (HER2) + breast cancer model. (A) HER2 expression percentages on BT474 cells, as analyzed by flow cytometry using a two-step protocol. (B) Number of reovirus genomic segment 4 (S4) copies in BT474 cells after reovirus infection. BT474 cells (200,000/well) were infected with reovirus multiplicities of infection (MOI) 10 or phosphate-buffered saline (PBS) (Mock) as a control. Samples (n=3) were harvested 24 hours after infection and the number of viral S4 copies was determined by quantitative reverse transcription PCR. (C) Design of experiment described in (D and E). Mice (n=6/group) with established BT474 tumors were intravenously (i.v.) injected with 5×10 6 human PBMCs, and thereafter intratumorally (i.t.) injected with reovirus (10 7 plaque-forming units (pfu)) on 2 consecutive days. After 4 days, mice received intraperitoneal (i.p.) injections of 12.5 µg CD3xHER2 bsAbs (CD3xHER2) or PBS as control. (D) Individual growth curves of BT474-bearing mice receiving indicated treatments. Lines indicate timing of injection with PBMCs (orange), reovirus (blue) or CD3xHER2 (red). (E) Average relative changes (±SEM) in tumor volume from start of CD3xHER2 bsAb treatment. Significance versus PBS on day 42 was calculated using one-way analysis of variance with Dunnett’s post hoc test. Significance level: **p<0.01.

Article Snippet: Female nonobese diabetic (NOD).Cg-Prkdc scid Il2rg tm1Wjl /SzJ (NSG) mice of 6 weeks old (Charles River Laboratories) were used for the BT474 model. BT474 tumors were orthotopically engrafted by injecting 5×10 6 cells in a volume of 100 μL 50:50 PBS/0.1% BSA:Growth Factor Reduced matrigel (Corning) in the fourth mammary fat pad of isoflurane-anesthesized mice.

Techniques: Expressing, Flow Cytometry, Infection, Saline, Control, Reverse Transcription, Injection

Journal: Oncotarget

Article Title: Inhibition of PI4K IIIα radiosensitizes in human tumor xenograft and immune-competent syngeneic murine tumor model

doi: 10.18632/oncotarget.22778

Figure Lengend Snippet: Tumor volume curves of each group; control, simeprevir alone, radiation alone, and simeprevir+radiation. Tumor growth was significantly delayed in the group treated with simeprevir plus radiation in BT474 breast cancer model (P < 0.05). No significant differences between control, simeprevir alone, and radiation alone were found.

Article Snippet: By injecting 5 × 10 6 BT474 cells into the hind limb of 6- to 8-week-old BALB/c athymic nude mice (Orient Bio, Inc., Sungnam, Korea), human breast cancer xenografts were established.

Techniques: Control

Journal: Oncotarget

Article Title: Inhibition of PI4K IIIα radiosensitizes in human tumor xenograft and immune-competent syngeneic murine tumor model

doi: 10.18632/oncotarget.22778

Figure Lengend Snippet: Migration and invasion potential of U251 cells and BT474 cells were assessed with wound healing assays (A) and modified Boyden chamber assay (B) , respectively. Stained cells were analyzed in representative fields (x100). Selective inhibition of PI4K IIIα using RNAi inhibited cell migration and invasion in both cell lines and similar results were found after treatment with 200 nM simeprevir. The inhibition of cell migration and invasion was more prominent after treatment with simeprevir and radiation. Bar and asterisk represent standard error and statistical significance of p<0.05 compared with control, respectively. Abbreviations. Sim = simeprevir

Article Snippet: By injecting 5 × 10 6 BT474 cells into the hind limb of 6- to 8-week-old BALB/c athymic nude mice (Orient Bio, Inc., Sungnam, Korea), human breast cancer xenografts were established.

Techniques: Migration, Modification, Boyden Chamber Assay, Staining, Inhibition, Control

Journal: Oncotarget

Article Title: Inhibition of PI4K IIIα radiosensitizes in human tumor xenograft and immune-competent syngeneic murine tumor model

doi: 10.18632/oncotarget.22778

Figure Lengend Snippet: Specific inhibition of PI3Kδ using RNA interference increased radiosensitivity of BT474 and MDA-MB-468 cells.

Article Snippet: By injecting 5 × 10 6 BT474 cells into the hind limb of 6- to 8-week-old BALB/c athymic nude mice (Orient Bio, Inc., Sungnam, Korea), human breast cancer xenografts were established.

Techniques: Inhibition